What is Universal SG Fluorescence Oligo System?
Current real-time PCR requires high cost to synthesize the labeled probe for each target, which limits wide application of real-time PCR. Universal SG fluorescence oligo system is uniquely designed, allowing its users to use only one Universal SG fluorescence oligo for detecting hundreds of different targets in real-time PCR. Universal SG fluorescence oligo system offers benefits of reducing cost as well as saving time and efforts on probe design and setting up PCR condition.
Universal SG Fluorescence Oligo System
- Uses One Universal SG Fluorescence Oligo to detect hundreds of different targets of your interest
- Significantly lowers the cost for detecting multiple targets in real-time PCR
: the more targets you detect with Universal SG Florescence Oligo, the more you reduce in cost
- Save your time and efforts on designing probe and setting up PCR condition
1. Target Binding
Real-time PCR is initiated with hybridization of primers and SG probe to the selected target sequence. The Tagging portion of the SG probe is a unique sequence and does not hybridize to any target sequences.
2. Extension & Cleavage
After Taq polymerase-mediated extension of primers, Taq polymerase having a 5’-exonuclease activity encounters the target-bound SG probe, and releases the Tagging portion of the SG probe.
3. Tagging Binding & Extension
The sequence of released Tagging portion is complementary to the Universal SG fluorescence template. As the Tagging portion is fully extended on the artificial SG template to create the Duplex, quenching is diminished and the fluorescent signal can be detected.